Oxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endothelium

About 3000 tons of beans are not used in human food due to hardening. Several studies on bean-derived bioactive peptides have shown potential treat some diseases, including those relying oxidative dysfunctions. We assessed the effects extracted from hardened bean Phaseolus vulgaris (PV) reactive oxygen species (ROS) and nitric oxide (NO) production, cytotoxic cytoprotective endothelial cells, oxidonitrergic-dependent vasodilating effects. Extract was composed by peptide fraction <3 kDa (PV3) common residue. PV3 sequences were obtained analyzed with bioinformatics. Human umbilical vein cells treated 10, 20, 30, 250 µg/mL PV3. Oxidative stress provoked 3% H2O2. Cytotoxicity evaluated MTT assay, whereas, ROS NO quantified using DHE DAF-FM fluorescent probes confocal microscopy. NO- endothelium-dependent isolated aortic rings. found 35 an average mass 1.14 kDa. There no cell deaths 10 20 μg/mL at 30 increased viability, while cytotoxicity observed only able protect stress. also release without causing death. It reduced relative production induced relied release. low-commercial-value displays little exerts antioxidant effects, whereas it increases